How do I wash an email list?

I have a list of about 5,000 opted in leads that were provided to me. I'm not sure of the age of these leads. Basically I want to wash them and verify that they are still valid and remove the ones that are not longer interested and opted out.

What would be the most effective and economical way to take my list and refine it to filter out the good leads from the leads that are no longer interested?

If I just send all of the leads an email asking them, I fear they would just be annoyed and request to be taken off the list. What is the best approach here?

use aweber and send emails, and check how many of them are beign opened.

I noticed over the last week or so many of the spam emails I recieved were asking for return reciepts. I guess tht would be a way but I have never done any email marketing.

Usman Nasir said:

use aweber and send emails, and check how many of them are beign opened.

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Right now my email list is just an excel file. Will Aweber allow me to dump my raw excel file directly into the aweber system?